Evaluation of Different Culture Media for Improvement in Bioinsecticides Production by Indigenous Bacillus thuringiensis and Their Application against Larvae of Aedes aegypti

Table 3

Toxic effect of Bt  𝑠𝑣2 on different media against IVth instars larvae of Aedes aegypti and comparative cost analysis for production of toxin.

Culture media (g/L)	LC50  ± SE (LCL-UCL)	LC90  ± SE (LCL-UCL)	Regression equation	Cost per liter in USD	Net difference* (in ratio)
LB medium (25)	4.02±0.26 (3.57–4.65)	7.59±0.64 (6.56–9.25)	𝑌=0.438+2.37𝑋	1.61	—
BN + pigeon pea (5)	1.57±0.09 (1.37–1.76)	3.20±0.17 (2.89–3.62)	𝑌=3.66+3.69𝑋	0.07	23
BN + soyabean (5)	3.17±0.16 (2.86–3.54)	5.94±0.37 (5.31–6.85)	𝑌=0.167+3.12𝑋	0.07	23

LC₅₀: lethal concentration that kills 50% of the exposed larvae, LC₉₀: lethal concentration that kills 90% of the exposed larvae, SE: standard error, and LCL-UCL: 95% upper and lower fiducial limits. LC₅₀ and LC₉₀ expressed in ppm.
*Ratio of cost of LB medium required for preparation of 1 L medium with the test medium.