An Efficient In Vitro Plantlet Regeneration from Shoot Tip Cultures of Curculigo latifolia, a Medicinal Plant
Table 1
Effect of different concentration of TDZ and IBA on percentage of explants producing shoots, shoot numbers, and shoot length after 10 and 14 weeks of culture.
TDZ (mgL−1)
IBA (mgL−1)
Mean
Week 10
Week 14
Explants producing shoots (%)
Number of shoots
Shoot length (cm)
Explants producing shoots (%)
Number of shoots
Shoot length (cm)
0
0
—
—
—
—
—
—
0
0.25
—
—
—
—
—
—
0
0.5
—
—
—
—
—
—
0.5
0
38.89cd
3.00ab
1.01bc
61.11bc
5.44b
2.03b
0.5
0.25
77.78a
3.08a
1.43a
83.33a
7.52a
2.71a
0.5
0.5
61.11b
2.5abc
1.22ab
66.67ab
5.5b
1.98bc
1
0
33.33d
1.5de
1.12ab
50.00bcd
2.75cd
1.78bcd
1
0.25
38.89cd
1.83cde
1.17ab
44.44cde
3.28c
1.65cde
1
0.5
27.78d
1.33ef
1.00bc
38.89def
2.06de
1.47def
1.5
0
44.44bcd
2.61abc
1.09abc
50.00bcd
5.56b
1.84bc
1.5
0.25
38.89cd
2.28abcd
0.85bc
61.11bc
6.25b
1.71bcde
1.5
0.5
55.56bc
2.67abc
0.88bc
66.67ab
3.27c
1.26fg
2
0
33.33d
2.17bcde
0.74cd
38.89def
3.22c
1.40ef
2
0.25
11.11e
0.67fg
0.45d
27.78ef
1.67e
1.05gh
2
0.5
0.00e
0.00g
0.00e
22.22f
1.67e
0.76h
-test
**
**
**
*
**
**
ns: nonsignificant; *significant at ; **significant at ; —: no response.