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The Scientific World Journal
Volume 2014 (2014), Article ID 275085, 9 pages
http://dx.doi.org/10.1155/2014/275085
Research Article

Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences

1Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of Korea
2DaejeonDaejeon Institute of National Forensic Service, 1524 Yuseongdae-ro, Yuseong-gu, Daejeon 305-348, Republic of Korea
3Department of Science Education, Chinju National University of Education, 3 Jinyangho-ro 369 gil, Jinju 660-756, Republic of Korea

Received 7 March 2014; Revised 29 April 2014; Accepted 29 April 2014; Published 28 May 2014

Academic Editor: Jaewoo Yoon

Copyright © 2014 Yu-Hoon Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Identification of insect species is an important task in forensic entomology. For more convenient species identification, the nucleotide sequences of cytochrome c oxidase subunit I (COI) gene have been widely utilized. We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagidae fly species collected in Korea. After DNA extraction from collected flies, PCR amplification and automatic sequencing of the whole COI sequence were performed. Obtained sequences were analyzed for a phylogenetic tree and a distance matrix. Our data showed very low intraspecific sequence distances and species-level monophylies. However, sequence comparison with previously reported sequences revealed a few inconsistencies or paraphylies requiring further investigation. To the best of our knowledge, this study is the first report of COI nucleotide sequences from Hydrotaea occulta, Muscina angustifrons, Muscina pascuorum, Ophyra leucostoma, Sarcophaga haemorrhoidalis, Sarcophaga harpax, and Phaonia aureola.