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The Scientific World Journal
Volume 2014 (2014), Article ID 279451, 12 pages
http://dx.doi.org/10.1155/2014/279451
Research Article

Antioxidant Capacity and Radical Scavenging Effect of Polyphenol Rich Mallotus philippenensis Fruit Extract on Human Erythrocytes: An In Vitro Study

1Department of Pharmacology, Faculty of Modern Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
2Department of Microbiology, Faculty of Modern Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
3Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 21005, India

Received 19 July 2014; Revised 27 October 2014; Accepted 4 November 2014; Published 26 November 2014

Academic Editor: Raffaella Sorrentino

Copyright © 2014 Mayank Gangwar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. D. Harman, “Aging: phenomena and theories,” Annals of the New York Academy of Sciences, vol. 854, pp. 1–7, 1998. View at Publisher · View at Google Scholar · View at Scopus
  2. S. R. J. Maxwell, “Prospects for the use of antioxidant therapies,” Drugs, vol. 49, no. 3, pp. 345–361, 1995. View at Publisher · View at Google Scholar · View at Scopus
  3. E. Niki, H. Shimaski, and M. Mino, Antioxidantism-Free Radical and Biological Defense, Gakkai Syuppn Center, Tokyo, Japan, 1994.
  4. C. A. Rice-Evans, N. J. Miller, P. G. Bolwell, P. M. Bramley, and J. B. Pridham, “The relative antioxidant activities of plant-derived polyphenolic flavonoids,” Free Radical Research, vol. 22, no. 4, pp. 375–383, 1995. View at Publisher · View at Google Scholar · View at Scopus
  5. M. Gerber, M.-C. Boutron-Ruault, S. Hercberg, E. Riboli, A. Scalbert, and M.-H. Siess, “Food and cancer: state of the art about the protective effect of fruits and vegetables,” Bulletin du Cancer, vol. 89, no. 3, pp. 293–312, 2002. View at Google Scholar · View at Scopus
  6. M. Gangwar, D. Kumar, R. Tilak et al., “Qualitative phytochemical characterization and antibacterial evaluation of glandular hairs of Mallotus philippinensis fruit extract,” Journal of Pharmacy Research, vol. 4, pp. 4214–4216, 2011. View at Google Scholar
  7. M. Gangwar, A. Dalai, A. Chaudhary, T. D. Singh, S. K. Singh, and G. Nath, “Study on activity of alcoholic extract of glands and hairs of fruits of Mallotus philippinensis in murine cestodal infection model,” International Journal of Pharmacy and Pharmaceutical Sciences, vol. 4, no. 1, pp. 643–645, 2012. View at Google Scholar · View at Scopus
  8. M. Gangwar, V. C. Verma, T. D. Singh, S. K. Singh, R. K. Goel, and G. Nath, “In-vitro scolicidal activity of Mallotus philippinensis (Lam.) Muell Arg. fruit glandular hair extract against hydatid cyst Echinococcus granulosus,” Asian Pacific Journal of Tropical Medicine, vol. 6, no. 8, pp. 595–601, 2013. View at Publisher · View at Google Scholar · View at Scopus
  9. R. Singh, K. C. Singhal, and N. U. Khan, “Antifilarial activity of Mallotus philippensis Lam. on Setaria cervie (Nematoda: Filarioidea) in vitro,” Indian Journal of Physiology and Pharmacology, vol. 41, no. 4, pp. 397–403, 1997. View at Google Scholar · View at Scopus
  10. M. Gangwar, R. K. Goel, and G. Nath, “Mallotus philippinensis Muell. Arg (Euphorbiaceae): ethnopharmacology and phytochemistry review,” BioMed Research International, vol. 2014, Article ID 213973, 13 pages, 2014. View at Publisher · View at Google Scholar
  11. J. D. Lambert, S. Sang, and C. S. Yang, “Possible controversy over dietary polyphenols: benefits vs risks,” Chemical Research in Toxicology, vol. 20, no. 4, pp. 583–585, 2007. View at Publisher · View at Google Scholar · View at Scopus
  12. R. Foncea, C. Carvajal, C. Almarza, and F. Leighton, “Endothelial cell oxidative stress and signal transduction,” Biological Research, vol. 33, no. 2, pp. 89–96, 2000. View at Google Scholar · View at Scopus
  13. A. Sofowora, “Screening plants for bioactive agents,” in Medicinal Plants and Traditional Medicinal in Africa, pp. 134–156, Spectrum Books, Sunshine House, Ibadan, Nigeria, 2nd edition, 1993. View at Google Scholar
  14. G. E. Trease and W. C. Evans, Pharmacognosy, Saunders, Philadelphia, Pa, USA, 15th edition, 2002.
  15. J. B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Chapman & Hall, New York, NY, USA, 3rd edition, 1998.
  16. C. K. Kokate, A. P. Purohit, and S. B. Gokhale, Pharmacognosy, vol. 35, Nirali Prakashan, Pune, India, 2006, pp. 133–525.
  17. V. L. Singleton and J. A. Rossi Jr., “Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents,” The American Journal of Enology and Viticulture, vol. 16, no. 3, pp. 144–158, 1965. View at Google Scholar
  18. D. K. Kajaria, M. Gangwar, A. K. Sharma, Y. B. Tripathi, J. S. Tripathi, and S. K. Tiwari, “Evaluation of in-vitro antioxidant capacity and reducing potential of polyherbal drug-Bharangyadi,” Ancient Science of Life, vol. 32, no. 1, pp. 24–28, 2012. View at Google Scholar
  19. A. K. Sharma, M. Gangwar, A. P. Chaturvedi, A. S. K. Sinha, and Y. B. Tripathi, “Comparative analysis of phenolic and flavonoid content of Jatropha Curcas Linn,” Plant Archives, vol. 12, no. 2, pp. 823–826, 2012. View at Google Scholar · View at Scopus
  20. A. Meda, C. E. Lamien, M. Romito, J. Millogo, and O. G. Nacoulma, “Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity,” Food Chemistry, vol. 91, no. 3, pp. 571–577, 2005. View at Publisher · View at Google Scholar · View at Scopus
  21. J. L. C. Lamaison and A. Carret, “Teneurs en principaux flavonoids des fleurs de Crataegus monogyna Jacq et de Crataegus laevigata (Piret DC) en fonction de la vegetation,” Plantes Medicinales et Phytotherapie, vol. 25, pp. 12–16, 1990. View at Google Scholar
  22. W. Brand-Williams, M. E. Cuvelier, and C. Berset, “Use of a free radical method to evaluate antioxidant activity,” LWT—Food Science and Technology, vol. 28, no. 1, pp. 25–30, 1995. View at Publisher · View at Google Scholar · View at Scopus
  23. L. L. Mensor, F. S. Menezes, G. G. Leitao et al., “Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method,” Phytotherapy Research, vol. 15, pp. 127–130, 2001. View at Google Scholar
  24. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Radical Biology and Medicine, vol. 26, no. 9-10, pp. 1231–1237, 1999. View at Publisher · View at Google Scholar · View at Scopus
  25. C. Beauchamp and I. Fridovich, “A mechanism for the production of ethylene from methional. The generation of the hydroxyl radical by xanthine oxidase,” The Journal of Biological Chemistry, vol. 245, no. 18, pp. 4641–4646, 1970. View at Google Scholar · View at Scopus
  26. O. I. Aruoma and B. Halliwell, “Action of hypochlorous acid on the antioxidant protective enzymes superoxide dismutase, catalase and glutathione peroxidase,” Biochemical Journal, vol. 248, no. 3, pp. 973–976, 1987. View at Google Scholar · View at Scopus
  27. E. Kunchandy and M. N. A. Rao, “Oxygen radical scavenging activity of curcumin,” International Journal of Pharmaceutics, vol. 58, no. 3, pp. 237–240, 1990. View at Publisher · View at Google Scholar · View at Scopus
  28. D. R. Janero, “Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury,” Free Radical Biology & Medicine, vol. 9, no. 6, pp. 515–540, 1990. View at Publisher · View at Google Scholar · View at Scopus
  29. H. Ohkawa, N. Ohishi, and K. Yagi, “Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction,” Analytical Biochemistry, vol. 95, no. 2, pp. 351–358, 1979. View at Publisher · View at Google Scholar · View at Scopus
  30. L. C. Green, D. A. Wagner, J. Glogowski, P. L. Skipper, J. S. Wishnok, and S. R. Tannenbaum, “Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids,” Analytical Biochemistry, vol. 126, no. 1, pp. 131–138, 1982. View at Publisher · View at Google Scholar · View at Scopus
  31. M. A. Vives, M. R. Infante, E. Garcia, C. Selve, M. Maugras, and M. P. Vinardell, “Erythrocyte hemolysis and shape changes induced by new lysine-derivate surfactants,” Chemico-Biological Interactions, vol. 118, no. 1, pp. 1–18, 1999. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Battistelli, R. de Sanctis, R. de Bellis, L. Cucchiarini, M. Dachà, and P. Gobbi, “Rhodiola rosea as antioxidant in red blood cells: ultrastructural and hemolytic behaviour,” European Journal of Histochemistry, vol. 49, no. 3, pp. 243–254, 2005. View at Google Scholar · View at Scopus
  33. A. K. Tiwari, “Imbalance in antioxidant defence and human diseases: multiple approach of natural antioxidants therapy,” Current Science, vol. 81, no. 9-10, pp. 1179–1187, 2001. View at Google Scholar · View at Scopus
  34. K.-J. Yeum, G. Aldini, H.-Y. Chung, N. I. Krinsky, and R. M. Russell, “The activities of antioxidant nutrients in human plasma depend on the localization of attacking radical species,” Journal of Nutrition, vol. 133, no. 8, pp. 2688–2691, 2003. View at Google Scholar · View at Scopus
  35. B. Halliwell and J. M. Gutteridge, Free Radicals in Biology and Medicine, Oxford University Press, Oxford, UK, 1998.
  36. A. Jerome-Morais, A. M. Diamond, and M. E. Wright, “Dietary supplements and human health: for better or for worse?” Molecular Nutrition and Food Research, vol. 55, no. 1, pp. 122–135, 2011. View at Publisher · View at Google Scholar · View at Scopus
  37. G. Bjelakovic and C. Gluud, “Surviving antioxidant supplements,” Journal of the National Cancer Institute, vol. 99, no. 10, pp. 742–743, 2007. View at Publisher · View at Google Scholar · View at Scopus
  38. O. K. Chun, A. Floegel, S. J. Chung, C. E. Chung, W. O. Song, and S. I. Koo, “Estimation of antioxidant intakes from diet and supplements in U.S. adults,” Journal of Nutrition, vol. 140, no. 2, pp. 317–324, 2010. View at Publisher · View at Google Scholar
  39. S. V. Jovanovic and M. G. Simic, “Antioxidants in nutrition,” Annals of the New York Academy of Sciences, vol. 899, pp. 326–334, 2000. View at Google Scholar · View at Scopus
  40. P. Mondon, L. Leclercq, and K. Lintner, “Evaluation of free-radical scavenger effects of Helianthus annus extracts using new ex vivo tripping methods,” Cosmetics, Aerosols & Toiletries in Australia, vol. 12, pp. 87–98, 1999. View at Google Scholar
  41. N. C. Cook and S. Samman, “Flavonoids—chemistry, metabolism, cardioprotective effects, and dietary sources,” Journal of Nutritional Biochemistry, vol. 7, no. 2, pp. 66–76, 1996. View at Publisher · View at Google Scholar · View at Scopus
  42. H. Li, Z. Wang, and Y. Liu, “Review in the studies on tannins activity of cancer prevention and anticancer,” Zhong Yao Cai, vol. 26, no. 6, pp. 444–448, 2003. View at Google Scholar · View at Scopus
  43. A. C. U. Lourens, D. Reddy, K. H. C. Başer, A. M. Viljoen, and S. F. van Vuuren, “In vitro biological activity and essential oil composition of four indigenous South African Helichrysum species,” Journal of Ethnopharmacology, vol. 95, no. 2-3, pp. 253–258, 2004. View at Publisher · View at Google Scholar · View at Scopus
  44. B. Havsteen, “Flavonoids, a class of natural products of high pharmacological potency,” Biochemical Pharmacology, vol. 32, no. 7, pp. 1141–1148, 1983. View at Publisher · View at Google Scholar · View at Scopus
  45. M. Wang, J. Li, M. Rangarajan et al., “Antioxidative phenolic compounds from sage (Salvia officinalis),” Journal of Agricultural and Food Chemistry, vol. 46, pp. 4869–4873, 1998. View at Google Scholar
  46. D. C. Garratt, The Quantitative Analysis of Drugs, vol. 3, Chapman & Hall, Tokyo, Japan, 1964.
  47. M. Korycka-Dahl and T. Richardson, “Photogeneration of superoxide anion in serum of bovine milk and in model systems containing riboflavin and amino acids,” Journal of Dairy Science, vol. 61, no. 4, pp. 400–407, 1978. View at Publisher · View at Google Scholar
  48. J. Robak and R. J. Gryglewski, “Flavonoids are scavengers of superoxide anions,” Biochemical Pharmacology, vol. 37, no. 5, pp. 837–841, 1988. View at Publisher · View at Google Scholar · View at Scopus
  49. B. Lipinski, “Hydroxyl radical and its scavengers in health and disease,” Oxidative Medicine and Cellular Longevity, vol. 2011, Article ID 809696, 9 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  50. L. W. Aurand, N. H. Boone, and G. G. Giddings, “Superoxide and singlet oxygen in milk lipid peroxidation,” Journal of Dairy Science, vol. 60, pp. 363–369, 1977. View at Google Scholar
  51. R. Saijo, G. Nonaka, and I. Nishioka, “Tannins and related compounds. LXXXVIII.: isolation and characterization of hydrolyzable tannins from Mallotus japonicus (THUNB.) MUELLER-ARG. and M. philippinensis (LAM.) MUELLER-ARG,” Chemical & Pharmaceutical Bulletin, vol. 37, no. 11, pp. 2940–2947, 1989. View at Google Scholar
  52. B. S. Taylor, Y.-M. Kim, Q. Wang, R. A. Shapiro, T. R. Billiar, and D. A. Geller, “Nitric oxide down-regulates hepatocyte-inducible nitric oxide synthase gene expression,” Archives of Surgery, vol. 132, no. 11, pp. 1177–1183, 1997. View at Publisher · View at Google Scholar · View at Scopus
  53. R. E. Huie and S. Padmaja, “The reaction of NO with superoxide,” Free Radical Research Communications, vol. 18, no. 4, pp. 195–199, 1993. View at Publisher · View at Google Scholar · View at Scopus
  54. S. Moncada, R. M. J. Palmer, and E. A. Higgs, “Nitric oxide: physiology, pathophysiology, and pharmacology,” Pharmacological Reviews, vol. 43, no. 2, pp. 109–142, 1991. View at Google Scholar · View at Scopus
  55. F. N. Ko, G. Hsiao, and Y. H. Kuo, “Protection of oxidative hemolysis by demethyldiisoeugenol in normal and β-thalassemic red blood cells,” Free Radical Biology & Medicine, vol. 22, no. 1-2, pp. 215–222, 1996. View at Publisher · View at Google Scholar · View at Scopus
  56. M. A. Ebrahimzadeh, S. Ehsanifar, and B. Eslami, “Sambucus ebulus elburensis fruits: a good source for antioxidants,” Pharmacognosy Magazine, vol. 4, no. 19, pp. 213–218, 2009. View at Google Scholar · View at Scopus
  57. Organization for Economic Co-operation and Development (OECD), OECD Guidelines for Testing of Chemicals-425 (Acute Oral Toxicity-Up and Down Regulation Procedure), 1948, http://www.oecd.org/chemicalsafety/risk-assessment/1948378.pdf.
  58. M. Gangwar, M. K. Gautam, S. Ghildiyal, G. Nath, and R. K. Goel, “Mallotus philippinensis Muell. Arg fruit glandular hairs extract promotes wound healing on different wound model in rats,” BMC Complementary and Alternative Medicine. In press.