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The Scientific World Journal
Volume 2014 (2014), Article ID 280146, 12 pages
http://dx.doi.org/10.1155/2014/280146
Research Article

Optimization of Aeration and Agitation Rate for Lipid and Gamma Linolenic Acid Production by Cunninghamella bainieri 2A1 in Submerged Fermentation Using Response Surface Methodology

1School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
2Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

Received 9 February 2014; Revised 29 September 2014; Accepted 29 September 2014; Published 31 December 2014

Academic Editor: Sami Aifa

Copyright © 2014 Normah Saad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Abdeshahian, M. G. Dashti, M. S. Kalil, and W. M. W. Yusoff, “Production of biofuel using biomass as a sustainable biological resource,” Biotechnology, vol. 9, no. 3, pp. 274–282, 2010. View at Publisher · View at Google Scholar · View at Scopus
  2. Q. Li, W. Du, and D. Liu, “Perspectives of microbial oils for biodiesel production,” Applied Microbiology and Biotechnology, vol. 80, no. 5, pp. 749–756, 2008. View at Publisher · View at Google Scholar · View at Scopus
  3. G. Venkata Subhash and S. Venkata Mohan, “Lipid accumulation for biodiesel production by oleaginous fungus Aspergillus awamori: influence of critical factors,” Fuel, vol. 116, pp. 509–515, 2014. View at Publisher · View at Google Scholar · View at Scopus
  4. P. Dey, J. Banerjee, and M. K. Maiti, “Comparative lipid profiling of two endophytic fungal isolates—Colletotrichum sp. and Alternaria sp. having potential utilities as biodiesel feedstock,” Bioresource Technology, vol. 102, no. 10, pp. 5815–5823, 2011. View at Publisher · View at Google Scholar · View at Scopus
  5. S. Fakas, S. Papanikolaou, A. Batsos, M. Galiotou-Panayotou, A. Mallouchos, and G. Aggelis, “Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina,” Biomass and Bioenergy, vol. 33, no. 4, pp. 573–580, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. H. Gema, A. Kavadia, D. Dimou, V. Tsagou, M. Komaitis, and G. Aggelis, “Production of γ-linolenic acid by Cunninghamella echinulata cultivated on glucose and orange peel,” Applied Microbiology and Biotechnology, vol. 58, no. 3, pp. 303–307, 2002. View at Publisher · View at Google Scholar · View at Scopus
  7. E. M. Taha, O. Omar, W. M. W. Yusoff, and A. A. Hamid, “Lipid biosynthesis in Cunninghamella bainieri 2A1 in N-limited and N-excess media,” Annals of Microbiology, vol. 60, no. 4, pp. 615–622, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. S. Papanikolaou and G. Aggelis, “Lipids of oleaginous yeasts. Part I: biochemistry of single cell oil production,” European Journal of Lipid Science and Technology, vol. 113, no. 8, pp. 1031–1051, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. N. Bati, E. G. Hammond, and B. A. Glatz, “Biomodification of fats and oils: trials with Candida lipolytica,” Journal of the American Oil Chemists' Society, vol. 61, no. 11, pp. 1743–1746, 1984. View at Publisher · View at Google Scholar · View at Scopus
  10. S. Bellou, A. Moustogianni, A. Makri, and G. Aggelis, “Lipids containing polyunsaturated fatty acids synthesized by zygomycetes grown on glycerol,” Applied Biochemistry and Biotechnology, vol. 166, no. 1, pp. 146–158, 2012. View at Publisher · View at Google Scholar · View at Scopus
  11. C. N. Economou, G. Aggelis, S. Pavlou, and D. V. Vayenas, “Modeling of single-cell oil production under nitrogen-limited and substrate inhibition conditions,” Biotechnology and Bioengineering, vol. 108, no. 5, pp. 1049–1055, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. C. N. Economou, G. Aggelis, S. Pavlou, and D. V. Vayenas, “Single cell oil production from rice hulls hydrolysate,” Bioresource Technology, vol. 102, no. 20, pp. 9737–9742, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. S. Fakas, M. Galiotou-Panayotou, S. Papanikolaou, M. Komaitis, and G. Aggelis, “Compositional shifts in lipid fractions during lipid turnover in Cunninghamella echinulata,” Enzyme and Microbial Technology, vol. 40, no. 5, pp. 1321–1327, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. S. Papanikolaou, M. Galiotou-Panayotou, S. Fakas, M. Komaitis, and G. Aggelis, “Lipid production by oleaginous Mucorales cultivated on renewable carbon sources,” European Journal of Lipid Science and Technology, vol. 109, no. 11, pp. 1060–1070, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. S. Fakas, M. Čertik, S. Papanikolaou, G. Aggelis, M. Komaitis, and M. Galiotou-Panayotou, “γ-Linolenic acid production by Cunninghamella echinulata growing on complex organic nitrogen sources,” Bioresource Technology, vol. 99, no. 13, pp. 5986–5990, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. G. Aggelis and J. Sourdis, “Prediction of lipid accumulation-degradation in oleaginous micro-organisms growing on vegetable oils,” Antonie van Leeuwenhoek, vol. 72, no. 2, pp. 159–165, 1997. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Thiry and D. Cingolani, “Optimizing scale-up fermentation processes,” Trends in Biotechnology, vol. 20, no. 3, pp. 103–105, 2002. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Bandaiphet and P. Prasertsan, “Effect of aeration and agitation rates and scale-up on oxygen transfer coefficient, kLa in exopolysaccharide production from Enterobacter cloacae WD7,” Carbohydrate Polymers, vol. 66, no. 2, pp. 216–228, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. D. Gao, J. Zeng, Y. Zheng, X. Yu, and S. Chen, “Microbial lipid production from xylose by Mortierella isabellina,” Bioresource Technology, vol. 133, pp. 315–321, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. D. Somashekar, G. Venkateshwaran, K. Sambaiah, and B. R. Lokesh, “Effect of culture conditions on lipid and gamma-linolenic acid production by mucoraceous fungi,” Process Biochemistry, vol. 38, no. 12, pp. 1719–1724, 2003. View at Publisher · View at Google Scholar · View at Scopus
  21. Z. Ruan, M. Zanotti, X. Wang, C. Ducey, and Y. Liu, “Evaluation of lipid accumulation from lignocellulosic sugars by Mortierella isabellina for biodiesel production,” Bioresource Technology, vol. 110, pp. 198–205, 2012. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Kendrick and C. Ratledge, “Desaturation of polyunsaturated fatty acids in Mucor circinelloides and the involvement of a novel membrane-bound malic enzyme,” European Journal of Biochemistry, vol. 209, no. 2, pp. 667–673, 1992. View at Publisher · View at Google Scholar · View at Scopus
  23. J. Folch, M. Lees, and G. H. Sloane-Stanley, “A simple method for the isolation and purification of total lipides from animal tissues,” The Journal of Biological Chemistry, vol. 226, no. 1, pp. 497–509, 1957. View at Google Scholar · View at Scopus
  24. M. G. Dashti, P. Abdeshahian, W. M. W. Yusoff, M. S. Kalil, and A. A. Hamid, “Repeated batch fermentation biotechnology for the biosynthesis of lipid and gamma-linolenic acid by Cunninghamella bainieri 2A1,” BioMed Research International, vol. 2014, Article ID 831783, 12 pages, 2014. View at Publisher · View at Google Scholar
  25. A. Chatzifragkou, S. Fakas, M. Galiotou-Panayotou, M. Komaitis, G. Aggelis, and S. Papanikolaou, “Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi,” European Journal of Lipid Science and Technology, vol. 112, no. 9, pp. 1048–1057, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Papanikolaou, P. Diamantopoulou, A. Chatzifragkou, A. Philippoussis, and G. Aggelis, “Suitability of low-cost sugars as substrates for lipid production by the fungus thamnidium elegans,” Energy and Fuels, vol. 24, no. 7, pp. 4078–4086, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. E. Zikou, A. Chatzifragkou, A. A. Koutinas, and S. Papanikolaou, “Evaluating glucose and xylose as cosubstrates for lipid accumulation and γ-linolenic acid biosynthesis of Thamnidium elegans,” Journal of Applied Microbiology, vol. 114, no. 4, pp. 1020–1032, 2013. View at Publisher · View at Google Scholar · View at Scopus
  28. A.-N. Vamvakaki, I. Kandarakis, S. Kaminarides, M. Komaitis, and S. Papanikolaou, “Cheese whey as a renewable substrate for microbial lipid and biomass production by Zygomycetes,” Engineering in Life Sciences, vol. 10, no. 4, pp. 348–360, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. P. Vijayaraghavan and S. G. P. Vincent, “Medium optimization for the production of fibrinolytic enzyme by Paenibacillus sp. IND8 using response surface methodology,” The Scientific World Journal, vol. 2014, Article ID 276942, 9 pages, 2014. View at Publisher · View at Google Scholar
  30. S. Bellou, A. Makri, D. Sarris et al., “The olive mill wastewater as substrate for single cell oil production by Zygomycetes,” Journal of Biotechnology, vol. 170, no. 1, pp. 50–59, 2014. View at Publisher · View at Google Scholar · View at Scopus
  31. G. Aggelis, “Two alternative pathways for substrate assimilation by Mucor circinelloides,” Folia Microbiologica, vol. 41, no. 3, pp. 254–256, 1996. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Fakas, A. Makri, M. Mavromati, M. Tselepi, and G. Aggelis, “Fatty acid composition in lipid fractions lengthwise the mycelium of Mortierella isabellina and lipid production by solid state fermentation,” Bioresource Technology, vol. 100, no. 23, pp. 6118–6120, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. C. Ratledge and J. P. Wynn, “The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms,” Advances in Applied Microbiology, vol. 51, pp. 1–52, 2002. View at Publisher · View at Google Scholar · View at Scopus
  34. A. B. Sitanggang, H.-S. Wu, S. S. Wang, and Y.-C. Ho, “Effect of pellet size and stimulating factor on the glucosamine production using Aspergillus sp. BCRC 31742,” Bioresource Technology, vol. 101, no. 10, pp. 3595–3601, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. I. B. Bajaj and R. S. Singhal, “Effect of aeration and agitation on synthesis of poly(γ-glutamic acid) in batch cultures of Bacillus licheniformis NCIM 2324,” Biotechnology and Bioprocess Engineering, vol. 15, no. 4, pp. 635–640, 2010. View at Publisher · View at Google Scholar · View at Scopus
  36. S. Papanikolaou, I. Chevalot, M. Galiotou-Panayotou, M. Komaitis, I. Marc, and G. Aggelis, “Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica,” Electronic Journal of Biotechnology, vol. 10, no. 3, pp. 425–435, 2007. View at Publisher · View at Google Scholar · View at Scopus
  37. E. Germain and T. Stephenson, “Biomass characteristics, aeration and oxygen transfer in membrane bioreactors: their interrelations explained by a review of aerobic biological processes,” Reviews in Environmental Science and Biotechnology, vol. 4, no. 4, pp. 223–233, 2005. View at Publisher · View at Google Scholar · View at Scopus
  38. Y. Chisti and U. J. Jauregui-Haza, “Oxygen transfer and mixing in mechanically agitated airlift bioreactors,” Biochemical Engineering Journal, vol. 10, no. 2, pp. 143–153, 2002. View at Publisher · View at Google Scholar · View at Scopus