Optimization of Aeration and Agitation Rate for Lipid and Gamma Linolenic Acid Production by Cunninghamella bainieri 2A1 in Submerged Fermentation Using Response Surface Methodology

Table 2

Experimental design and test results of lipid and GLA produced by Cunninghamella bainieri 2A1 in the submerged bioreactor.

Run			Lipid concentration (g/L)	GLA concentration (mg/L)
1	−1	1	4.23	567.5
2	0	0	4.53	573.12
3	0	−1.41	1.23	144.0
4	0	0	4.60	602.82
5	1	−1	2.35	322.65
6	0	0	4.18	539.09
7	1.41	0	4.74	747.72
8	0	0	4.62	591.0
9	1	1	3.95	621.0
10	−1.41	0	3.01	433.5
11	0	0	4.31	573.12
12	0	1.41	4.34	567.36
13	−1	−1	1.125	145.0

: aeration rate (vvm); : agitation rate (rpm).