Optimization of Aeration and Agitation Rate for Lipid and Gamma Linolenic Acid Production by Cunninghamella bainieri 2A1 in Submerged Fermentation Using Response Surface Methodology

Table 4

Analysis of variance for the quadratic polynomial model of biomass production by Cunninghamella bainieri 2A1.

Source	Polynomial coefficients	Sum of squares	DF	Mean square	value	Prob >
Model		1.39	5	0.28	335.28	<0.0001^*
Intercept	2.47		1
	0.11	0.11	1	0.11	127.19	<0.0001^*
	0.33	0.87	1	0.87	1045.02	<0.0001^*
	−0.081	0.046	1	0.046	54.94	0.0001^*
	−0.23	0.38	1	0.38	461.40	<0.0001^*
	−0.066	0.018	1	0.018	21.18	0.0025^*
Residual			7
Lack of Fit			3		4.16	0.1010
Pure Error			4

Statistically significant at 99% probability level.
: aeration rate (vvm); : agitation rate (rpm); and : the quadratic terms; : the interaction term.
= 0.9958.