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The Scientific World Journal
Volume 2014, Article ID 345794, 8 pages
http://dx.doi.org/10.1155/2014/345794
Research Article

An Effective Protocol for Micropropagation of Edible Bamboo Species (Bambusa tulda and Melocanna baccifera) through Nodal Culture

1Institute of Bioresources and Sustainable Development, Takyelpat, Imphal, Manipur 795001, India
2Centre of Advanced Study in Life Sciences, Manipur University, Imphal, Manipur 795003, India
3Department of Biotechnology, The University of Burdwan, Golapbag More, West Bengal 713104, India
4Department of Biochemistry, University of Yaoundé I, BP 812, Yaounde, Cameroon

Received 3 March 2014; Revised 1 May 2014; Accepted 7 May 2014; Published 21 May 2014

Academic Editor: Amparo Latorre

Copyright © 2014 Sayanika Devi Waikhom and Bengyella Louis. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

High demand for edible bamboo shoots of Bambusa tulda and Melocanna baccifera in many Asian ethnic groups has led to the need for developing intensive bamboo farming. To achieve this, in vitro regeneration of bamboo plantlets is needed due to the long and irregular bamboo flowering cycle and scarcity of bamboo seeds. An effective protocol for plantlets regeneration in B. tulda and M. baccifera from nodal explants following validation of the species using the sequence of trnL-F intergenic spacer region is described. Effective axillary bud breaking was achieved at 3 mg/L of 6-benzylaminopurine (BAP) in MS medium. Importantly, combining 2 mg/L of kinetin (Kn) with 3 mg/L of BAP produced a synergistic effect for shoot multiplication in B. tulda and M. baccifera. Under optimized conditions in half-strength MS medium supplemented with 3 mg/L of indole-3-butyric acid (IBA), 10 mg/L of coumarin, and 3% sucrose, profuse production of dark-brown rhizome in B. tulda and abundant rooting (81.67%, , ) for M. baccifera within 30 days were achieved. The established protocol and the validation of the reported species at the molecular level will be of help to stakeholders in edible bamboo trade to conserve gene-pool and increase productivity.