Genetic Variability of Aspergillus flavus Isolates from a Mississippi Corn Field

Table 1

Aspergillus flavus isolates collected in Elizabeth, MS, from 2006 through 2009, UV fluorescence and ELISA aflatoxin detection (+/−), norB-cypA amplicon fragment size, and omtA haplotype frequency.

Sources	Time period	AF (+)	AF (−)	norB-cypA patterns	omtA haplotypes (frequency)
Soil	Jan.–Apr. 2006	140, 161, 163–172	159, 160, 162, 173	4^b; 12^c	H1 (10)^d, H6 (1), H14 (1), H17 (1), H49 (2)
	May–Oct. 2006	93–99	90–92	4^b; 6^c	H1 (5), H7 (1), H11 (1), H49 (1)
	Jan.–Apr. 2007	56–58, 60, 76–80, 141, 143–146, 148, 149, 153–155	59, 81, 82, 142, 147, 150–152, 156	7^b; 21^c	H1 (15), H16 (1), H19 (1), H49 (6)
	May–Oct. 2007	101, 105, 107, 110, 115, 123, 138, 139	100, 108	2^b; 8^c	H1 (7), H8 (1), H18 (1), H49 (1)
	Jan.–Apr. 2008	61, 62, 64, 83–88, 157, 158, 175–177, 180	63, 65–67, 174, 178, 179	6^b; 14^c	H1 (15), H49 (2)
	May–Oct. 2008	111, 113, 116, 117, 120, 121, 127, 136	132	3^b; 6^c	H1 (4), H5 (1), H15 (1), H21 (1), H49 (1)
	Jan.–Apr. 2009	55, 73	50–54, 68–72, 74, 75, 89	8^b; 7^c	H1 (2), H2 (1), H49 (2)
Above ground^a	May–Oct. 2006	124, 128, 134, 137	102, 106, 114, 122, 131, 135	6^b; 4^c	H1 (3), H3 (1), H4 (1), H7 (2), H49 (2)
	May–Oct. 2008	104, 109, 112, 118, 125, 126, 129, 133	103, 119	2^b; 8^c	H1 (6), H20 (1), H22 (1), H23 (1), H49 (1)
Plant debris	Jan.–Apr. 2006	1–8	9–14	7^b; 7^c	H1 (4), H12 (1)
	Jan.–Apr. 2007	19, 22, 24–26, 28, 29	16–18, 20, 21, 23, 27, 30	15^b	H8 (2), H9 (1), H10 (1)
	Jan.–Apr. 2008	34, 36–38, 40–45	31–33, 35, 39	11^b; 4^c	H8 (2), H13 (2)
Combined		105	66	74^b, 97^c	118^e

Above ground corresponding to isolates from silk, tassel, ear, and insect.
^b300 bp fragment, deletion pattern I.
^c800 bp fragment, deletion pattern II.
^dNumber of isolates belonging to the indicated haplotype.
^eNumber of isolates examined for omtA amplicon.