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The Scientific World Journal
Volume 2014, Article ID 357052, 12 pages
http://dx.doi.org/10.1155/2014/357052
Research Article

Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane

1Federal University of Pernambuco (UFPE/CCB/Genética), 50670-420 Recife, PE, Brazil
2ABC Medical School, 09060-650 São Paulo, SP, Brazil
3Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil
4Institute of Molecular Biosciences, Goethe University Frankfurt am Main, 60438 Frankfurt am Main, Germany

Received 14 January 2014; Revised 20 April 2014; Accepted 21 April 2014; Published 2 June 2014

Academic Editor: Juan J. Loor

Copyright © 2014 Roberta Lane de Oliveira Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material showing the quality of the total RNAs from sugarcane roots under drought stress (FIGURE S1 - A), the amplicons of expected size for each gene used in the study, displaying primer specificity as required for RT-qPCR amplification (FIGURE S1 - B), and the melting curves showing a single peak for six potential reference genes (αTUB; GAPDH; H1; SAMDC; UBQ and 25S rRNA) and four targets (PFPα1; AS; PIP1-1 and ACC oxidase).

Supplementary Material (FIGURE S2) showing standard curves using a dilution series (100, 10-1, 10-2, 10-3 e 10-4) of six potential reference genes (αTUB; GAPDH; H1; SAMDC; UBQ and 25S rRNA) for sugarcane roots under drought stress and the respective curves for four targets (PFPα1; AS; PIP1-1 and ACC oxidase).

Supplementary Material (TABLE S2) based on MIQE checklist for authors, reviewers, and editors.

Supplementary Material (TABLE S1) showing Cq values of potential reference genes for gene expression normalization (RT-qPCR) with cDNAs from sugarcane roots under drought stress (24 h of continuous dehydration).

  1. Supplementary Materials