The Scientific World Journal

Research Article

Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane

Table 1

Potential reference genes and target genes with the individual accession numbers, annotations, and primer sequences.
Gene (accession numbers)Source*Predicted functionDescriptionPrimer sequences (forward/reverse)
Reference genes    
  TUB (CN607271)dbESTStructural constituent of cytoskeletonAlpha-tubulin(F) CCATTGGCAAGGAGATTGTT
(R) TCCACCAACTGCATTGAAGA
 GAPDH (CA254672)dbESTGlycolysis, gluconeogenesisGlyceraldehyde 3 phosphate dehydrogenase(F) GGTTCACTTGAAGGGTGGTG
(R) TGAGGTGTACCTGTCCTCGTT
 H1 (CA116806)[23]Chromatin condensationHistone H1(F) CGCACACGCACACTGAAAG
(R) CGGTGGCCATGATCAAAAA
 SAMDC (CA127376)[23]Polyamine and triamine biosynthesis S-Adenosylmethionine decarboxylase(F) TGCTGCTGAAGACGCTGTTG
(R) TCGCCTTCAAAGCAGTGTAGAAC
 UBQ (CA077378)dbESTProtein degradationUbiquitin(F) ACCGAAGGTTGCATTCAAGAC
(R) GGGTTTGGGTCCGTTAGAAG
 25S rRNA (BQ536525)dbESTTranslation25S ribosomal RNA(F) GCAGCCAAGCGTTCATAG
(R) CGGCACGGTCATCAGTAG
Target genes    
 PFP 1 (XM_004973200.1)dbESTCarbohydrate metabolism, glycolysisPyrophosphate fructose-6-phosphate 1-phosphotransferase alpha subunit(F) TTATGAGTTGCTGCGAGAGAAG
(R) TATCTCAATGTCGCCCATGTAG
 AS (FM212633.1)dbESTAsparagine synthesisGlutamine-dependent asparagine synthetase(F) CCAGAGAACACACCCACAAC
(R) ATGCCACACTAGGACCTCCA
 PIP1-1 (CF572112)dbESTIntegral to membrane, water transportPlasma membrane intrinsic protein 1-1(F) GTTCCTATCCTTGCCCCACT
(R) AGGCGTGATCCCTGTTGTAG
 ACC oxidase (TC127289)SoGIEthylene biosynthesis1-Aminocyclopropane-1-carboxylate oxidase(F) GGGACCTCTTGCAGATAATGTC
(R) CTCTGGCAATGGTCCATAGAA
Databases: dbEST (NCBI; http://www.ncbi.nlm.nih.gov/) and Gene Index (SoGI; http://compbio.dfci.harvard.edu/tgi/); Rodrigues et al. [23]. TUB: alpha-tubulin; GAPDH: glyceraldehyde 3 phosphate dehydrogenase; H1: histone H1; SAMDC: S-adenosylmethionine decarboxylase; UBQ: ubiquitin; 25S rRNA: 25S ribosomal RNA; PFP 1: pyrophosphate fructose-6-phosphate 1-phosphotransferase alpha subunit (EC 2.7.1.90); AS: glutamine-dependent asparagine synthetase (EC 6.3.5.4); PIP1-1: plasma membrane intrinsic protein; ACC oxidase: 1-aminocyclopropane-1-carboxylate oxidase (EC 1.14.17.4).