Validation of Novel Reference Genes for Reverse Transcription Quantitative Real-Time PCR in Drought-Stressed Sugarcane

Table 3

Expression stability values for sugarcane candidate calculated by NormFinder and BestKeeper software.

Ranking	NormFinder analysis		BestKeeper analysis
	Gene name	𝑀	Gene name	CV ± SD
1	𝛼TUB	0.11	H1	(1.06±0.26)
2	H1	0.16	𝛼TUB	(1.06±0.29)
3	GAPDH	0.19	GAPDH	(1.33±0.31)
4	25S rRNA	0.28	25S rRNA	(3.89±0.55)
5	UBQ	0.31	UBQ	(2.04±0.50)
6	SAMDC	0.33	SAMDC	(2.53±0.57)

𝑀: average expression stability value; CV: coefficient of variance; SD: standard deviation; 𝛼TUB: alpha-tubulin; H1: histone H1; GAPDH: glyceraldehyde 3 phosphate dehydrogenase; 25S rRNA: 25S ribosomal RNA; UBQ: ubiquitin; SAMDC: S-adenosylmethionine decarboxylase.