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The Scientific World Journal
Volume 2014, Article ID 380850, 6 pages
http://dx.doi.org/10.1155/2014/380850
Research Article

Evaluation of Cytotoxicity and Genotoxicity of Acacia aroma Leaf Extracts

1Área de Microbiología, Fac de Qca, Bqca y Fcia, UNSL, San Luis, Argentina
2Área de Biología, Fac de Qca, Bqca y Fcia, UNSL, San Luis, Argentina
3Área de Farmacognosia, Fac de Qca, Bqca y Fcia, UNSL, San Luis, Argentina
4Departamento de Microbiología e Inmunología, Fac de Cs Ex. Fco-Qco y Nat., UNRC, Río Cuarto, Córdoba, Argentina

Received 16 July 2014; Accepted 15 September 2014; Published 28 October 2014

Academic Editor: Daiji Endoh

Copyright © 2014 C. M. Mattana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Acacia aroma, native plant from San Luis, Argentina, is commonly used as antiseptic and for healing of wounds. The present study was conducted to investigate the in vitro cytotoxicity and genotoxicity of hot aqueous extract (HAE) and ethanolic extract (EE) of A. aroma. The cytotoxic activity was assayed by neutral red uptake assay on Vero cell. Cell treatment with a range from 100 to 5000 μg/mL of HAE and EE showed that 500 μg/mL and 100 μg/mL were the maximum noncytotoxic concentrations, respectively. The CC50 was 658 μg/mL for EE and 1020 μg/mL for HAE. The genotoxicity was tested by the single-cell gel electrophoresis comet assay. The results obtained in the evaluation of DNA cellular damage exposed to varied concentrations of the HAE showed no significant genotoxic effect at range of 1–20 mg/mL. The EE at 20 mg/mL showed moderate genotoxic effect related to the increase of the DNA percentage contained in tail of the comet; DNA was classified in category 2. At concentrations below 5 mg/mL, the results of cytotoxicity and genotoxicity of aqueous and ethanolic extracts of Acacia aroma guarantee the safety at cell and genomic level. However further studies are needed for longer periods including animal models to confirm the findings.