Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability
Effect of temperature on lipase activity and thermostability. (a) Effect of temperature on lipase activity. Lipase activity was detected in 50 mmol PBS, pH 8.0 under different temperatures (25–55°C; temperature interval is 5°C). (b) Thermal stability of three lipases. Lipases were incubated at 40°C for different durations and residual activities were measured under standard assay conditions (50 mmol PBS, pH 8.0, and 40°C). The activity at 0 h (nonincubated) was taken as 100%. The values represent the means of three independent experiments (mean ± SD). WT, V94W, and I100F were shown as triangle (▲), circle (●), and square (■).