Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability
Effect of pH on lipase activity. Lipase activity was detected at 40°C in 50 mmol buffers of various pH values (5.5–9.0; pH value is 0.5). The values represent the means of three independent experiments (mean ± SD). WT, V94W, and I100F were shown as triangle (▲), circle (●), and square (■).