Marker-Assisted Selection for Recognizing Wheat Mutant Genotypes Carrying HMW Glutenin Alleles Related to Baking Quality

Table 1

STS primers used to amplify HMW glutenin subunits.

Forward PCR primers (5′………3′)	Reverse PCR primers (5′………3′)	References
P1 = 5′ ACCTTATCCATGCAAGCTACC 3′	P2 = 5′ CATGGCAGCCGACCGGCCAAC 3′	Smith et al. [22]
P3 = 5′ GTTGGCCGGTCGGCTGCCATG 3′	P4 = 5′ TGGAGAAGTTGGATAGTACC 3′	Smith et al. [22]
P5 = 5′ GCCTAGCAACCTTCACAATC 3′	P6 = 5′ GAAACCTGCTGCGGACAAG 3′	Ahmad [8]
P7 = 5′ AGCCTAGCAACCTTCAC 3′	P8 = 5′ AGACATGCAGCACATACC 3′	De Bustos et al. [15]
P9 = 5′ CTAACTCGCCGTGCACA 3′	P10 = 5′ AGCTAAGGTGCATGCATG 3′	De Bustos et al. [15]
P11 = 5′ CTCGTCCCTATAAAAGCCTAGT 3′	P12 = 5′ GAGACATGCAGCACATACT 3′	De Bustos et al. [15]
P13 = 5′ AGCTAAGGTGCATGCATG 3′	P14 = 5′ CTAACTCGCCGTGCACA 3′	De Bustos et al. [15]
P15 = 5′ ATGGCTAAGCGGTTAGTCCTC 3′	P16 = 5′ GCATTGTCGGCCAGCCAGTGA 3′
P17 = 5′ GCATTGTCGGCCAGCCAGTGA 3′	P18 = 5′ ACAAGGGCAACAAGGTCAGCA 3′
P19 = 5′ GCATTGTCGGCTAGCCAGTGA 3′	P20 = 5′ ATGGCTAAGCGGCTGGTCCTC 3′