Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
Figure 7
SDS-PAGE analysis of proteins extracted from biomass of Chlorella sorokiniana: 50% ammonium sulfate fractions of control (lanes 1 and 3) and 40 mg·L−1 selenate-exposed (lanes 2 and 4) cultures, 60% ammonium sulfate fractions of control (lane 5), 40 mg·L−1 selenate-exposed (lane 6) cultures, 40% ammonium sulfate fractions of control (lane 7), and 40 mg·L−1 selenate-exposed (lane 8) cultures. All lanes were extracted from cells of Chlorella sorokiniana after 120 h of cultivation. Lanes 1 to 4 origin from initial cultivation, while lanes 5 to 8 belong to repeated cultivation. Lanes 1 and 2 were loaded with 15 μg of proteins, lane 3 with 20 μg, lane 4 with 10 μg, while lanes 5 to 8 contained 7.5 μg of proteins each. S = protein ladder (molecular weight marker).
