Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method
Figure 1
Chromatograms from the analysis of lycorine in H. littoralis. (a) Standard lycorine 6.25 μg/mL. (b) Anther extract at 400 μg/mL. (c) Extract of callus culture obtained with 4.5 μM 2,4-D and 0 μM BAP at 1000 μg/mL. Column: Eclipse Plus reversed-phase C-18 (250 mm × 4.6 mm i.d., 5 μm); mobile phase: acetonitrile-1% aqueous ammonium acetate buffer (15 : 85 v/v); flow rate: 1.0 mL/min; wavelength: 325 nm.
