Kinetics of Mushroom Tyrosinase and Melanogenesis Inhibition by N-Acetyl-pentapeptides
Figure 3
Lineweaver-Burk double reciprocal plot and determination of inhibition constants for Ac-P1 to Ac-P4 (a to d) on mushroom tyrosinase. The data include mean values of , and the inverse of the absorption increased at 475 nm per minute, in 3 independent tests with various concentrations of L-DOPA as a substrate. The reaction was observed in the presence of 0, 0.1, and 0.2 mg/mL of Ac-P1 to Ac-P4.