Expression, Purification, and Functional Analysis of Novel RelE Operon from X. nematophila

Figure 4

Endogenous toxicity assay. All the experiments were performed in triplicate, and mean values were used to show the results in percentage (%) growth at different time intervals. Bacterial growth was monitored by determining optical density at 600 nm in the presence of arabinose. (◆) JSL strain [WT (wild type) strain + empty vector (control)]; (▲) JSL3 strain [WT (wild type) strain + Xn-relE toxin]; and (■) JSL4 strain [WT (wild type) + operon (Xn-relE + Xn-relEAT)].