The Scientific World Journal / 2014 / Article / Fig 4

Research Article

Micropropagation of an Exotic Ornamental Plant, Calathea crotalifera, for Production of High Quality Plantlets

Figure 4

Development of high quality plantlet from shoot bud explants of Calathea crotalifera on multiplication media (MS medium with 3.5 mg/L BAP and 1.0 mg/L NAA) and rooting media (MS medium with 1.0 mg/L NAA). (a) Induction of microshoots from incised shoot bud explants. (b) High quality plantlets with dark green leaves in multiplication medium supplemented with 1 g/L activated charcoal. (c) Plantlets with light green leaves without necrosis problem on multiplication medium supplemented with 1 g/L L-glutamine. (d) Plantlets with high multiple shoots induction. (e) Induction of healthy root in rooting media supplemented with 1.0 mg/L NAA. (f) Roots induction on rooting media supplemented with 1.0 mg/L IBA. (g) and (h) Healthy plantlets ready to be acclimatized. (i) Development of successfully acclimatized plantlets.

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