Micropropagation of an Exotic Ornamental Plant, Calathea crotalifera, for Production of High Quality Plantlets

Table 3

Effects of different concentrations and combinations of cytokinin and auxin on shoot production from two different sources of explants in yellow C. Crotalifera (CCY).

BAP (mg/L)	Kinetin (mg/L)	NAA (mg/L)	Number of shoots		Height of shoot (cm)		Percentage of leaf (%)
			Apical	Axillary	Apical	Axillary	Apical	Axillary
2.5	—	0.5	0.70 ± 0.19^ab	1.80 ± 0.37^a	1.82 ± 0.18^A	2.04 ± 0.15^A	0.00^a	79.70^a
2.5	—	1.0	1.40 ± 0.24^abcde	2.40 ± 0.40^abc	2.11 ± 0.11^B	2.32 ± 0.04^AB	0.00^a	83.10^a
3.0	—	0.0	2.20 ± 0.37^defg	3.40 ± 0.24^abcd	2.08 ± 0.18^B	3.35 ± 0.02^DE	0.00^a	83.36^a
3.0	—	0.5	2.00 ± 0.44^defg	3.40 ± 0.24^abcd	2.11 ± 0.06^B	3.21 ± 0.04^DE	0.00^a	81.00^a
3.0	—	1.0	1.80 ± 0.58^cdefg	3.60 ± 0.24^bcd	2.19 ± 0.07^BC	3.51 ± 0.04^E	6.80^c	92.54^bc
3.5	—	0.0	2.40 ± 0.58^efg	3.60 ± 0.51^bcd	2.21 ± 0.03^BC	3.60 ± 0.15^E	9.70^d	99.60^e
3.5	—	0.5	2.60 ± 0.24^ef	4.00 ± 0.45^cd	2.32 ± 0.04^BC	4.46 ± 0.06^F	4.24^b	100.00^e
3.5	—	1.0	2.80 ± 0.20^f	5.60 ± 0.51^e	2.40 ± 0.04^C	5.44 ± 0.06^G	4.10^b	97.90^de
4.0	—	1.0	1.80 ± 0.20^cdefg	4.40 ± 0.24^de	2.18 ± 0.05^BC	4.68 ± 0.10^F	0.00^a	94.00^bcd
5.0	—	1.0	0.80 ± 0.37^abc	3.40 ± 0.51^abcd	2.30 ± 0.04^BC	3.25 ± 0.09^DE	0.00^a	83.00^a
6.0	—	1.0	0.40 ± 0.24^a	2.00 ± 0.84^ab	2.25 ± 0.04^BC	2.61 ± 0.14^BC	0.00^a	81.10^a
—	1.5	1.0	1.20 ± 0.37^abcd	2.20 ± 0.86^ab	2.24 ± 0.04^BC	3.20 ± 0.31^DE	0.00^a	83.20^a
—	2.5	1.0	1.40 ± 0.24^abcde	2.60 ± 0.51^abc	2.25 ± 0.02^BC	3.50 ± 0.42^E	0.00^a	81.80^a
—	3.5	1.0	1.60 ± 0.24^bcdef	2.80 ± 0.58^abcd	2.31 ± 0.03^BC	2.90 ± 0.29^DE	0.00^a	96.10^cde
—	4.5	1.0	1.60 ± 0.24^bcdef	3.40 ± 0.51^abcd	2.26 ± 0.04^BC	3.68 ± 0.14^E	0.00^a	91.10^b

Mean ± SE, n = 10. Different letters indicate significant differences between media at P = 0.05.