Micropropagation of an Exotic Ornamental Plant, Calathea crotalifera, for Production of High Quality Plantlets

Table 4

Effects of different concentrations and combinations of cytokinin and auxin on shoot production from two different sources of explants in red C. Crotalifera (CCR).

BAP (mg/L)	Kinetin (mg/L)	NAA (mg/L)	Number of shoots		Height of shoot (cm)		Percentage of leaf (%)
			Apical	Axillary	Apical	Axillary	Apical	Axillary
2.5	—	0.5	1.00 ± 0.00^abc	1.80 ± 0.20^a	1.80 ± 0.20^A	2.13 ± 0.03^A	0.00^a	81.10^a
2.5	—	1.0	1.00 ± 0.00^abc	2.20 ± 0.20^a	1.90 ± 0.11^AB	2.25 ± 0.02^AB	0.00^a	83.10^ab
3.0	—	0.0	0.90 ± 0.24^abc	2.00 ± 0.00^a	2.22 ± 0.09^CDEF	3.64 ± 0.10^D	4.30^b	83.36^ab
3.0	—	0.5	1.20 ± 0.37^abcd	2.40 ± 0.40^a	2.11 ± 0.03^BCDE	3.63 ± 0.10^D	0.00^a	83.66^ab
3.0	—	1.0	1.20 ± 0.37^abcd	2.20 ± 0.37^a	2.31 ± 0.08^DEF	4.47 ± 0.16^E	6.80^c	91.54^cd
3.5	—	0.0	1.60 ± 0.24^cd	3.60 ± 0.40^bc	2.28 ± 0.07^DEF	4.49 ± 0.23^E	9.70^d	99.60^f
3.5	—	0.5	1.60 ± 0.24^cd	3.80 ± 0.37^bc	2.35 ± 0.05^EF	4.63 ± 0.17^EF	4.74^b	100.00^f
3.5	—	1.0	2.00 ± 0.32^d	4.40 ± 0.24^c	2.40 ± 0.04^F	4.85 ± 0.12^F	4.30^b	98.80^ef
4.0	—	1.0	1.40 ± 0.24^bcd	3.40 ± 0.51^b	2.04 ± 0.05^ABCD	3.06 ± 0.16^C	0.00^a	93.70^de
5.0	—	1.0	0.60 ± 0.24^ab	2.00 ± 0.32^a	1.99 ± 0.06^ABC	3.24 ± 0.07^C	0.00^a	83.00^ab
6.0	—	1.0	0.40 ± 0.24^a	1.60 ± 0.24^a	1.96 ± 0.12^ABC	2.31 ± 0.06^AB	0.00^a	81.10^a
—	1.5	1.0	1.20 ± 0.20^abcd	2.00 ± 0.00^a	2.07 ± 0.04^ABCD	3.16 ± 0.05^C	0.00^a	83.26^ab
—	2.5	1.0	1.40 ± 0.24^bcd	2.00 ± 0.32^a	2.04 ± 0.04^ABCD	3.22 ± 0.03^C	0.00^a	81.86^a
—	3.5	1.0	0.60 ± 0.24^ab	1.80 ± 0.37^a	2.16 ± 0.05^BCDEF	2.56 ± 0.12^B	0.00^a	85.00^ab
—	4.5	1.0	1.40 ± 0.24^bcd	1.80 ± 0.20^a	2.09 ± 0.06^BCDE	3.19 ± 0.12^C	0.00^a	88.10^bc

Mean ± SE, n = 10. Different letters indicate significant differences between media at P = 0.05.