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The Scientific World Journal
Volume 2014, Article ID 457380, 9 pages
http://dx.doi.org/10.1155/2014/457380
Research Article

Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2

1Laboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil
2Laboratory of Molecular Biology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil
3Department of Morphology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, Brazil
4Laboratory of Neuroanatomy, Department of Morphology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, Brazil
5Laboratory of Neurochemical Studies, Department of Physiology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, Brazil

Received 1 July 2014; Accepted 7 December 2014; Published 29 December 2014

Academic Editor: Yunfeng Lin

Copyright © 2014 Eudes Euler de Souza Lucena et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4. Cells cultivated only with conditioned medium acquired a glial phenotype. Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs.