Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development
Figure 3
Phenotypic effects of overexpression of full-length, N-terminal, or C-terminal TIMP-1 constructs. Following injection, photographs were taken of representative embryos at stage 30. (a) Control (uninjected) embryos were phenotypically normal. Microinjection of T1FL (b) and T1N (c) constructs both resulted in normal anterior (head) development but truncated posterior axes. (d) Microinjection of T1C constructs resulted in lack of head structures, other head defects, and neural tube closure failure. Panel (a) magnification is lower than in (b), (c), and (d). Embryos are 1 mm in diameter.
