Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development
Figure 5
Effect of overexpression of full-length, N-terminal, or C-terminal TIMP-1 on mRNA levels of MMP inhibitors. Semiquantitative RT-PCR analysis was used to measure changes in mRNA levels of (a) TIMP-2, (b) TIMP-3, and (c) RECK at stage 30, following microinjection of 4 ng of TIMP-1 full-length (T1FL), N-terminal (T1N), or C-terminal (T1C) constructs into X. laevis embryos at the 1-cell stage. In each case mRNA levels were measured relative to Ef1α. The results are presented as mean ± standard error from 3 independent experiments. * and **, all versus control (CONT; uninjected) embryos, as analyzed by one-way ANOVA and Dunnett’s post hoc test.
