Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development

Figure 6

Effect of overexpression of full-length, N-terminal, or C-terminal TIMP-1 on mRNA levels of MMPs. Semiquantitative RT-PCR analysis was used to measure changes in mRNA levels of (a) MMP-2, (b) MMP-9, and (c) MT1-MMP at stage 30, following microinjection of 4 ng of TIMP-1 full-length (T1FL), N-terminal (T1N), or C-terminal (T1C) constructs into X. laevis embryos at the 1-cell stage. In each case mRNA levels were measured relative to Ef1α. The results are presented as mean ± standard error from 3 independent experiments. *𝑃<0.05 and **𝑃<0.01, all versus control (CONT; uninjected) embryos, as analyzed by one-way ANOVA and Dunnett’s post hoc test.

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