Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development
Figure 7
Zymography demonstrated altered levels of active MMP-2 and MMP-9 following overexpression of TIMP-1 constructs. 4 ng of mRNA coding for full-length (T1FL), N-terminal (T1N), or C-terminal (T1C) constructs was injected into X. laevis embryos at the 1-cell stage and protein was isolated from stage 30 embryos. Gelatin zymography was used to measure changes in (a) MMP-2 and (b) MMP-9 activity. Zymogram (top) is representative of one experiment where the bright brand represents the active forms (63 and 84 kDa) of MMP-2 and MMP-9, respectively. Graphs represent quantification of above zymogram, and data is presented as mean ± standard error from 3 independent experiments. * and **, all versus control (CONT; uninjected) embryos, as analyzed by one-way ANOVA and Dunnett’s post hoc test.
