Functional Characterization of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) N- and C-Terminal Domains during Xenopus laevis Development

Figure 8

Reverse zymography demonstrating full-length and N-terminal, but not C-terminal, TIMP-1 can directly inhibit MMP activity. 4 ng of mRNA coding for full-length (T1FL), N-terminal (T1N), or C-terminal (T1C) TIMP-1 constructs was injected into X. laevis embryos at the 1-cell stage and protein was isolated from stage 30 embryos. MMP-inhibitory activity is represented by dark bands. White arrows indicate the location of the expected inhibitory band of the T1FL (26 KDa) and T1N (18 KDa) constructs, respectively. Black arrow represents position of T1C (12 KDa) construct, which cannot inhibit MMP activity as suggested by the light band.