Optimization and Validation of Indirect ELISA Using Truncated TssB Protein for the Serodiagnosis of Glanders amongst Equines

<table>Specific immune reactivity of recombinant TssB protein in western blot. Lanes TssB and M: amido black stained recombinant TssB protein and protein molecular weight marker, respectively, upon transfer on PVDF membrane. Lanes 1–14: Glanders positive equine serum, Lanes: 15–18: negative control serum, Lanes 19–22: melioidosis positive human serum. Equine disease control serum: Lanes 23–25: <i>Streptococcus equi</i> infection (strangles); Lane 26–28: <i>Streptococcus zooepidemicus</i> infection; Lane 29-30: and <i>Pseudomonas aeruginosa</i> infection.</table>

The Scientific World Journal

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Figure 3

Figure 3: Optimization and Validation of Indirect ELISA Using Truncated TssB Protein for the Serodiagnosis of Glanders amongst Equines 