Hydrogen Sulfide Increases Nitric Oxide Production and Subsequent S-Nitrosylation in Endothelial Cells

Figure 1

NaHS increases the expression and serine 1177 phosphorylation of eNOS. (a) ECs pretreated by diluting concentrations of NaHS (1 μM, 10 μM, 50 μM, and 100 μM) for 2 h. (b) ECs treated with NaHS (50 μM) for 0.5, 2, and 12 h. Blotted membranes were separately hybridized with eNOS and peNOSS1177 antibodies. ((c) and (d)) Relative folds of protein levels shown as means ± S.E. compared to control. Statistical significance (^∗𝑃<0.05; ^∗∗𝑃<0.01) analyzed using Fisher’s LSD.

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