Hydrogen Sulfide Increases Nitric Oxide Production and Subsequent S-Nitrosylation in Endothelial Cells
Figure 4
Detection of protein S-nitrosylation. (a) Scheme representing the procedures of modified biotin switch. Biotin-labeled lysates were subjected to SDS-PAGE without any reducing agents in the buffers. (b) ECs lysate (100 μg) treated with NaHS (50 μM) for 0.5, 2, and 12 h separated by SDS-PAGE and the blotted membranes were hybridized with streptavidin-HRP. Triangle indicates proteins with increased S-nitrosylation. Arrow head indicates proteins with decreased S-nitrosylation.
