Research Article

Hydrogen Sulfide Increases Nitric Oxide Production and Subsequent S-Nitrosylation in Endothelial Cells

Figure 5

Investigation of H2S biosynthesis under the treatment of NO. (a) ECs which treated NO donor, NOC-18 (250 μM), for 0.5, 2, and 12 h were subjected to western blot analysis with CSE, CBS, and 3-MST antibodies. (b) The statistic data showed that no significance ( ) was observed between treatments. (c) NBD-SCN was applied to detect cellular H2S level specifically. (d) Fluorescent signals were calculated by flow cytometry.
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