Amino Acid Sequence and Structural Comparison of BACE1 and BACE2 Using Evolutionary Trace Method

Figure 2

ET sequence compared to the seed sequences of BACE1 (P56817) and BACE2 (Q9Y5Z0). The domains within BACE1 and BACE2 sequences are marked with the coloured thick lines. Red and blue lines are for the N-terminal and C-terminal domains, respectively, whereas the green lines marked the interdomain regions within the structures. Amino acid residues for the flap region are marked within the box. Conserved residues are shown as their specific amino acid in the ET sequences, while group-specific residues are shown as alphabet “X.” Neutral residues are marked as “-” in the ET sequence. The amino acid numbering of BACE1 and BACE2 sequences is according to their 3D structures obtained from PDB file, 1FKN, and 2EWY, respectively. The starting amino acid residues are highlighted in red. Amino acid residues that are involved in the ligand binding sites of BACE1 and BACE2 are marked with asterisk. Red coloured asterisks are binding sites that are only for BACE1, while blue coloured asterisks are the ligand binding sites that are only for BACE2.