Overexpression of a Vesicle Trafficking Gene, OsRab7, Enhances Salt Tolerance in Rice

Figure 1

Expression of the OsRab7 gene in transgenic rice plants. (a) Real-time quantitative PCR analysis in transgenic plants. Total RNA was extracted from transgenic plant, and wild-type plants. Actin was used as an internal control. (b) OsRab7 expression in transgenic plants was determined by semiquantitative RT-PCR analysis. (c) a SDS-PAGE showed the protein content in the samples. Equal concentrations of protein extracts from the rice samples were separated by SDS-PAGE. (d) Western blot analysis of transgenic rice plants. All lanes were run with equal amounts of the protein extracts from the rice samples.