Research Article
Hematology of Wild Caught Dubois’s Tree Frog Polypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)
Table 1
Hematological parameters observed in adult males and females of Polypedates teraiensis.
| | Hematological parameters | Sex | | Male | Female | | X ± SD (range) | X ± SD (range) |
| | RBC count* | (0.58–0.61) | (0.61–0.65) | | Haemoglobin conc. (%) | (5.8–6.1) | (5.5–6.2) | | Packed cell volume (%) | (49.5–52.0) | (50.5–52.5) | | Mean corpuscular volume (µ³) | (819.67–866.66) | (803.07–833.33) | | Mean corpuscular haemoglobin (Pg) | (96.72–105.17) | (90.76–105.76) | | Mean corpuscular haemoglobin conc. (%) | (11.71–11.95) | (11.3–12.77) | | RBC, length (µm)* | (15.5–18.5) | (18.0–21.5) | | RBC, breadth (µm)* | (8.8–9.1) | (8.2–8.9) | | Length/breadth of RBC | (1.77–2.10) | (2.02–2.41) | | Surface area of erythrocyte (µm²) | (107.60–126.98) | (121.52–191.35) | | Length of RBC’s nucleus (µm) | (5.9–6.5) | (5.9–9.5) | | Breadth of RBC’s nucleus (µm) | (4.9–5.1) | (4.5–6.7) | | Length/breadth of RBC’s nucleus | (1.18–1.27) | (1.16–1.52) | | Surface area of RBC’s nucleus (µm²) | (23.01–25.85) | (20.7–49.64) | | WBC count (number of WBC/mm³) | (11.8–12.5) | (11.5–12.9) | | Neutrophil (%)* | (22.0–25.0) | (25.0–27.6) | | Lymphocyte (%) | (53.1–58.0) | (54.6–55.6) | | Basophil (%) | (3.2–3.8) | (1.9–4.2) | | Eosinophil (%) | (10.0–12.5) | (9.8–10.2) | | Monocyte (%) | (5.9–7.1) | (5.0–6.0) | | Neutrophil/lymphocyte ratio* | (0.37–0.45) | (0.45–0.50) | | Diameter of neutrophil (µm) | (9.0–11.5) | (10.0–11.5) | | Area occupied by neutrophil (µm²) | (63.62–103.81) | (78.55–103.81) | | Diameter of large lymphocyte (µm)* | (7.5–11.5) | (7.5–12.0) | | Area occupied by large lymphocyte (µm²) | (44.15–121.31) | (44.15–113.04) | | Diameter of small lymphocyte (µm) | (4.0–6.0) | (4.1–5.9) | | Area occupied by small lymphocyte (µm²) | (12.56–28.26) | (13.19–27.32) | | Diameter of basophil (µm) | (9.0–13.5) | (9.5–13.5) | | Area occupied by basophil (µm²)* | (63.58–143.06) | (70.84–143.06) | | Diameter of eosinophil (µm)* | (10.0–12.0) | (9.5–13.5) | | Area occupied by eosinophil (µm²) | (78.50–113.04) | (70.84–143.06) | | Diameter of monocyte (µm) | (10.0–12.0) | (10.0–12.0) | | Area occupied by monocyte (µm²) | (78.50–113.04) | (78.50–113.04) |
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: mean; SD: standard deviation; Pg: pictogram. Difference in values between males and females statistically significant.
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