Syntheses of Isoxazoline-Carbocyclic Nucleosides and Their Antiviral Evaluation: A Standard Protocol
Table 2
Cytotoxicity and antiviral activity of compounds 14a,b and 16a,b in HEL cell cultures.
Compound
MCCa (μg/mL)
MICb (μg/mL)
HSV-1 (KOS)
HSV-2 (G)
VV
VSV
HSV-1 TK-ACVr
14aA
80
>16
>16
>16
>16
>16
14aB
>400
>400
>400
>400
>400
>400
14aC
80
>16
>16
>16
>16
>16
14aD
400
>80
>80
>80
>80
>80
14bA
16
>3.2
>3.2
>3.2
>3.2
>3.2
14bB
>400
>400
>400
>400
>400
>400
14bC
80
>16
>16
>16
>16
>16
14bD
16
>3.2
>3.2
>3.2
>3.2
>3.2
16aU
>400
>400
>400
>400
>400
>400
16aT
>400
>400
>400
>400
>400
>400
16bU
400
>80
>80
>80
>80
>80
16bT
400
>80
>80
>80
>80
>80
Brivudine
>400
0.128
400
48
>400
>400
Ribavirin
>400
>400
48
240
>400
>400
Acyclovir
>400
0.64
0.64
>400
>400
400
Ganciclovir
>400
0.096
0.16
>100
>100
12
Minimum cytotoxic concentration, required to cause a microscopically detectable alteration of normal cell morphology.
bMinimum inhibitory concentration required to reduce virus-induced cytopathogenicity by 50%.
