Syntheses of Isoxazoline-Carbocyclic Nucleosides and Their Antiviral Evaluation: A Standard Protocol

Table 3

Cytotoxicity and antiviral activity of compounds 14a,b and 16a,b in HeLa cell cultures.

Compound	MCC^a (μg/mL)	MIC^b (μg/mL)
		VSV	CV	RSV
14aA	80	>16	>16	>16
14aB	400	>80	>80	>80
14aC	≥16	>16	>16	>16
14aD	80	>16	>16	>16
14bA	≥80	>80	>80	>80
14bB	≥80	>80	>80	>80
14bC	≥16	>16	>16	>16
14bD	≥80	>80	>80	>80
16aU	400	>80	>80	>80
16aT	≥80	>80	>80	>80
16bU	400	>80	>80	>80
16bT	≥80	>80	>80	>80
Brivudin	>400	>400	>400	>400
Ribavirin	>400	48	80	48
(S)-DHPA	>400	>400	>400	>400

Minimum cytotoxic concentration, required to cause a microscopically detectable alteration of normal cell morphology.
^bMinimum inhibitory concentration required to reduce virus-induced cytopathogenicity by 50%.