Manipulating Ratio Spectra for the Spectrophotometric Analysis of Diclofenac Sodium and Pantoprazole Sodium in Laboratory Mixtures and Tablet Formulation
Table 1
Regression parameters obtained for the determination of diclofenac (DCL) and pantoprazole (PAN) using the proposed methods.
Parameters
Ratio spectra peak to peak measurement
Ratio derivative method
Mean centering of ratio spectra
DCL
PAN
DCL
PAN
DCL
PAN
Wavelength(s) (in nm)
318.0 and 251.0
318.0 and 251.0
326.0
337.0
318.0
318.0
Calibration range (μg/mL)
2.0–24.0
2.0–20.0
2.0–24.0
2.0–20.0
2.0–24.0
2.0–20.0
Slope
0.5507
0.0187
0.0351
0.0243
0.4260
0.1668
Intercept
0.1112
0.0045
−0.0048
0.0039
−0.0071
0.0125
Correlation coefficient ()
0.9981
0.9977
0.9998
0.9996
0.9998
0.9991
LOD (μg/mL)
0.583
0.597
0.605
0.688
0.560
0.543
LOQ (μg/mL)
1.765
1.811
1.832
2.085
1.696
1.645
LOD: limit of detection; LOQ: limit of quantitation.
