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The Scientific World Journal
Volume 2014, Article ID 507351, 7 pages
http://dx.doi.org/10.1155/2014/507351
Research Article

Effects of Dimethylaminoethanol and Compound Amino Acid on D-Galactose Induced Skin Aging Model of Rat

1Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, 253 GongYe Middle Avenue, Guangzhou, Guangdong 510280, China
2Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Medical School, Qingdao University, Qingdao, Shandong 266003, China
3Department of Dermatology, Huang-Si Aesthetic Surgery Hospital, Beijing 100120, China
4Center for Advanced Orthopedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
5Institute of Plastic and Reconstructive Surgery, Weifang Medical University, Shandong 261042, China

Received 25 April 2014; Revised 1 July 2014; Accepted 1 July 2014; Published 14 July 2014

Academic Editor: Oliver Distler

Copyright © 2014 Su Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A lasting dream of human beings is to reverse or postpone aging. In this study, dimethylaminoethanol (DMAE) and compound amino acid (AA) in Mesotherapy were investigated for their potential antiaging effects on D-galactose induced aging skin. At 18 days after D-gal induction, each rat was treated with intradermal microinjection of saline, AA, 0.1% DMAE, 0.2% DMAE, 0.1% DMAE + AA, or 0.2% DMAE + AA, respectively. At 42 days after treatment, the skin wound was harvested and assayed. Measurement of epidermal and dermal thickness in 0.1% DMAE + AA and 0.2% DMAE + AA groups appeared significantly thicker than aging control rats. No differences were found in tissue water content among groups. Hydroxyproline in 0.1% DMAE + AA, 0.2% DMAE + AA, and sham control groups was much higher than all other groups. Collagen type I, type III, and MMP-1 expression was highly upregulated in both 0.1% DMAE + AA and 0.2% DMAE + AA groups compared with aging control. In contrast, TIMP-1 expression levels of various aging groups were significantly reduced when compared to sham control. Coinjection of DMAE and AA into target tissue has marked antiaging effects on D-galactose induced skin aging model of rat.