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The Scientific World Journal
Volume 2014, Article ID 510627, 15 pages
Research Article

Optimization of Large-Scale Culture Conditions for the Production of Cordycepin with Cordyceps militaris by Liquid Static Culture

1The Engineering and Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang, Guizhou 550025, China
2Institute of Biology, Guizhou Academy of Sciences, Guiyang, Guizhou 550009, China
3Institute of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
4Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11442, Saudi Arabia

Received 14 February 2014; Accepted 8 April 2014; Published 23 June 2014

Academic Editor: Rajesh Jeewon

Copyright © 2014 Chao Kang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cordycepin is one of the most important bioactive compounds produced by species of Cordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.