Effect of Codon Optimisation on the Production of Recombinant Fish Growth Hormone in Pichia pastoris
Figure 4
Production of recombinant oFGH and nFGH in Pichia pastoris. The yeast was cultured for 72 hrs with induction each 12 hrs with 1% absolute methanol. The supernatant samples were collected at 12, 24, 36, 48, 60, and 72 hrs and recombinant protein concentration was measured by FGH ELISA. The results showed that the production of oFGH was significantly higher () than nFGH in one litre culture whereas the total yeast cell-mass was approximately similar (two-tailed paired t-test).
