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The Scientific World Journal
Volume 2014 (2014), Article ID 516069, 12 pages
http://dx.doi.org/10.1155/2014/516069
Research Article

Development and Optimization of Polymeric Self-Emulsifying Nanocapsules for Localized Drug Delivery: Design of Experiment Approach

1MM College of Pharmacy, MM University, Mullana, Ambala 133207, India
2Government College of Pharmacy, Rohru, Shimla 171207, India

Received 23 July 2014; Accepted 22 September 2014; Published 24 November 2014

Academic Editor: Sanjula Baboota

Copyright © 2014 Jyoti Wadhwa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of the present study was to formulate polymeric self-emulsifying curcumin nanocapsules with high encapsulation efficiency, good emulsification ability, and optimal globule size for localized targeting in the colon. Formulations were prepared using modified quasiemulsion solvent diffusion method. Concentration of formulation variables, namely, (oil), (polymeric emulsifier), and (adsorbent), was optimized by design of experiments using Box-Behnken design, for its impact on mean globule size () and encapsulation efficiency () of the formulation. Polymeric nanocapsules with an average diameter of 100–180 nm and an encapsulation efficiency of 64.85 ± 0.12% were obtained. In vitro studies revealed that formulations released the drug after 5 h lag time corresponding to the time to reach the colonic region. Pronounced localized action was inferred from the plasma concentration profile ( 200 ng/mL) that depicts limited systemic absorption. Roentgenography study confirms the localized presence of carrier (0–2 h in upper GIT; 2–4 h in small intestine; and 4–24 h in the lower intestine). Optimized formulation showed significantly higher cytotoxicity (IC50 value 20.32 μM) in HT 29 colonic cancer cell line. The present study demonstrates systematic development of polymeric self-emulsifying nanocapsule formulation of curcumin for localized targeting in colon.