Optimization of Reflux Conditions for Total Flavonoid and Total Phenolic Extraction and Enhanced Antioxidant Capacity in Pandan (Pandanus amaryllifolius Roxb.) Using Response Surface Methodology

Table 2

The result of experimental and predicted values of reflux extraction of TF, TP, and AC.

Run	𝑋1	𝑋2	𝑋3	TF (mg/g DW)		TP (mg/g DW)		AC%
				Experimental values	Predicted values	Experimental values	Predicted values	Experimental values	Predicted values
1	10.52	50	30	0.55	0.56	3.12	3.18	44.70	44.80
2	80	70	20	1.52	1.50	6.24	6.20	83.20	83.37
3	50	50	16.84	1.16	1.15	5.55	5.50	70.40	70.42
4 (C)	50	50	30	1.33	1.26	5.94	5.90	75.80	74.48
5	80	70	40	1.74	1.70	6.58	6.55	87.50	87.36
6	20	30	20	0.67	0.68	3.79	3.82	52.00	52.19
7	50	76.32	30	1.40	1.39	6.06	6.06	80.00	80.10
8 (C)	50	50	30	1.22	1.26	5.84	5.90	73.00	74.48
9	80	30	40	1.44	1.43	6.10	6.16	79.40	79.81
10	20	30	40	0.71	0.71	3.82	3.85	53.10	52.98
11 (C)	50	50	30	1.31	1.26	5.89	5.90	75.30	74.48
12	50	50	43.16	1.35	1.33	5.95	5.91	75.80	75.60
13 (C)	50	50	30	1.27	1.26	6.02	5.97	74.20	74.48
14	50	23.68	30	1.15	1.17	5.89	5.83	72.70	73.10
15	20	70	20	0.81	0.79	3.99	3.93	53.80	53.43
16 (C)	50	50	30	1.28	1.26	5.94	5.90	74.50	74.48
17	89.48	50	30	1.38	1.40	6.11	6.14	80.50	80.61
18 (C)	50	50	30	1.21	1.26	5.85	5.90	73.80	74.48
19	20	70	40	0.88	0.90	4.10	4.12	54.90	55.82
20	80	30	20	1.37	1.32	6.00	5.98	78.80	77.49

(C): central point; 𝑋₁: MC; 𝑋₂: ET; 𝑋₃: LS ratio.