Lens Extrusion from Laminin Alpha 1 Mutant Zebrafish
Figure 2
Collagen IV aggregates are seen in lama1 a69/a69 mutant lens fibers. Immunofluorescent confocal microscopy showing collagen IV protein expression at 60 hpf. Eye from a wild type zebrafish embryo (a, b) showing normal distribution of collagen IV in the lens capsule at this stage. Eye from a lama1 a69/a69 mutant embryo showing downregulation of collagen IV expression in the lens capsule, while Collagen IV retention is seen in the lens fibers (c, d arrowheads). Collagen IV: green; DNA/Draq5: blue. lc: lens capsule. Scale bar = 35 m.
