Lens Extrusion from Laminin Alpha 1 Mutant Zebrafish
Figure 4
Laminin and BIGH3 expression downregulates in the developing cornea of lama1 a69/a69 mutant zebrafish. immunofluorescent confocal microscopy showing normal expression and distribution of laminin at 60 hpf in wild type embryos (a, b). Zebrafish lama1 a69/a69 mutants show downregulation of laminin in lens capsule (e) and discontinuous laminin staining in the developing cornea ((f) arrowheads). BIGH3 costaining with lens fiber cell specific marker ZL1 shows normal distribution at 60 hpf, in wild type embryos (c, d). Zebrafish lama1 a69/a69 mutant embryos show downregulation of corneal BIGH3 (h) and ZL1 positive cells were detected anterior to the anatomical boundary of the eye ((g) arrowheads). Laminin: red; (a, b, e, f), ZL1: red (c, d, g, h); BIGH3: green (c, d, g, h); Draq5: blue. f: lens fiber cells; ce: corneal epithelium; lc: lens capsule. Scale bar = 35 m.
