RSM Based Optimization of Chemical and Enzymatic Transesterification of Palm Oil: Biodiesel Production and Assessment of Exhaust Emission Levels
Table 4
Response surface quadratic model analysis of variance (ANOVA) for enzymatic transesterification of palm oil.
Source
df
SS (MS)d
SS (MS)e
value ( value)d
value ( value)e
Model
14
2666.17 (190.44)
2923.96 (208.85)
27.40 (<0.0001)
588.71 (<0.0001)
-enzyme concentration
1
1093.29 (1093.29)
226.00 (226.00)
157.33 (<0.0001)
637.04 (<0.0001)
-reaction time
1
28.38 (28.38)
120.55 (120.55`)
4.08 (0.0615)
339.79 (<0.0001)
-reaction temperature
1
4.07 (4.07)
2.69 (2.69)
0.59 (0.4558)
7.59 (0.0148)
-alcohol : oil molar ratio
1
63.73 (63.73)
12.62 (12.62)
9.17 (0.0085)
35.56 (<0.0001)
1
89.78 (89.78)
7.56 (7.56)
12.92 (0.0027)
21.32 (0.0003)
1
60.45 (60.45)
6.50 (6.50)
8.70 (0.0099)
18.33 (0.0007)
1
77.00 (77.00)
0.42 (0.42)
11.08 (0.0046)
1.19 (0.2924)
1
13.51 (13.51)
1.69 (1.69)
1.94 (0.1836)
4.76 (0.0454)
1
24.26 (24.26)
0.16 (0.16)
3.49 (0.0814)
0.45 (0.5121)
1
3.52 (3.52)
0.49 (0.49)
0.51 (0.4878)
1.38 (0.2582)
1
49.60 (49.60)
0.055 (0.055)
7.14 (0.0174)
0.16 (0.6993)
1
110.22 (110.22)
2.13 (2.13)
15.86 (0.0012)
5.99 (0.0271)
1
5.34 (5.34)
1.95 (1.95)
0.77 (0.3945)
5.50 (0.0331)
1
3.73 (3.73)
52.88 (52.88)
0.54 (0.4750)
149.05 (<0.0001)
Residual
15
104.24 (6.95)
5.32 (0.35)
Lack of fit
10
83.11 (8.31)
4.81 (0.48)
1.97 (0.2358)
4.68 (0.0511)
Pure error
5
21.13 (4.23)
0.51 (0.10)
Cor total
29
2770.41
2929.28
SS (MS) = sum of squares (mean square). Model d = represents quadratic model based on experimental results of A.n. lipase catalyzed transestrification of under-study feedstock. Model e = represents quadratic model based on experimental results of NOVOZYME-435 catalyzed transestrification of under-study feedstock.
