Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in Iris. lactea var. chinensis Roots under Cadmium, Lead, and Salt Stress Conditions

Table 2

Primer sequences and amplicon characteristics for each of the seven reference genes.

Name	Primer sequence (forward/reverse primer)	Size (bp)	𝑇𝑚 (°C)	𝐸 (%)	𝑅^2
UBC	5′-TCTCGCTTGTCCGGTTTGTG-3′	224	88.0	1.965	0.9993
	5′-ACCTTGGGTGGCTTGAATGG-3′
TUBLIN	5′-TTACCGTCAACTATTCCACCCA-3′	214	87	1.905	0.9991
	5′-CAGCAACGAACCCAAACCAGAT-3′
EIF-5A	5′-GGATGAGGAGCACCACTTCG-3′	110	90.5	1.912	0.9997
	5′-GGCGGTTCTTGATGACGATG-3′
EF1α	5′-CCATTTCTGGATTTGAGGGTGA-3′	133	86.5	2.016	0.9958
	5′-AGTCGAAGAGGCTTGTCGGTAG-3′
EF1b	5′-ATCTTCTGACCAGGAGTTACAT-3′	115	83.3	2.006	0.9997
	5′-TACCACCTAGCAACATTGAC-3′
ACTIN	5′-CTCAACCCGAAGGCAAACAGAG-3′	216	87.0	1.966	0.9994
	5′-CGCAAGGTCCAGACGGAGAATA-3′
HIS	5′-GGCTCGTACCAAGCAAACTG-3′	134	89.4	1.929	0.9993
	5′-TTCCAGGACGGTAACGATGA-3′