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The Scientific World Journal
Volume 2014, Article ID 536723, 6 pages
http://dx.doi.org/10.1155/2014/536723
Research Article

Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A

1Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul 136-703, Republic of Korea
2Department of Parasitology, Kangwon National University College of Medicine, Chuncheon, Kangwon-do 200-701, Republic of Korea
3College of Bionano Technology, Gachon Bionano Research Institute, Kyungwon University, Seongnam-si, Kyeonggi-do 461-701, Republic of Korea
4Department of Laboratory Medicine, College of Medicine, Korea University, Guro Hospital, Guro 2 Dong, Guro Gu, Seoul 152-703, Republic of Korea

Received 22 July 2014; Accepted 10 October 2014; Published 8 December 2014

Academic Editor: Nicola Micale

Copyright © 2014 Jin Woo Jang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2–37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2–66.0%). High correlation of measured parasitaemia (, ) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients.