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The Scientific World Journal
Volume 2014, Article ID 564573, 21 pages
http://dx.doi.org/10.1155/2014/564573
Research Article

Significance of Algal Polymer in Designing Amphotericin B Nanoparticles

1PDMCOP, Bahadurgarh, Haryana 124507, India
2Department of Pharmaceutical Sciences, Jamia Hamdard, New Delhi 110062, India
3School of Pharmacy, Faculty of Applied Medical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India
4Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India

Received 7 July 2014; Revised 1 September 2014; Accepted 15 September 2014; Published 12 November 2014

Academic Editor: Rizwan Hasan Khan

Copyright © 2014 Saurabh Bhatia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Development of oral amphotericin B (AmB) loaded nanoparticles (NPs) demands a novel technique which reduces its toxicity and other associated problems. Packing of AmB in between two oppositely charged ions by polyelectrolyte complexation technique proved to be a successful strategy. We have developed a novel carrier system in form of polyelectrolyte complex of AmB by using chitosan (CS) and porphyran (POR) as two oppositely charged polymers with TPP as a crosslinking agent. Initially POR was isolated from Porphyra vietnamensis followed by the fact that its alkali induced safe reduction in molecular weight was achieved. Formulation was optimized using three-factor three-level (33) central composite design. High concentration of POR in NPs was confirmed by sulfated polysaccharide (SP) assay. Degradation and dissolution studies suggested the stability of NPs over wide pH range. Hemolytic toxicity data suggested the safety of prepared formulation. In vivo and in vitro antifungal activity demonstrated the high antifungal potential of optimized formulation when compared with standard drug and marketed formulations. Throughout the study TPP addition did not cause any significant changes. Therefore, these experimental oral NPs may represent an interesting carrier system for the delivery of AmB.